Principle of the oral fluid Test

Oral fluid test has occupied most of the drug testing centers as it is easy to carry out the procedure. This article actively discusses about the principle of oral fluid test. Oral fluid sample used for Oralert fluid drug screen, Oralert drug test, Oralert testing for drugs, Oralert oral testing, Oratect screening for drugs.

The Multi Saliva Drug Test or Oral Fluid test is under the category of immunoassay test. It is based on the principle of competitive binding of drug metabolites to the specific antibody. Drugs which are present in the oral fluid specimen will compete in opposition to their own drug conjugate for binding spots on their specific antibody.

At some stage in testing, a part of the oral fluid specimen will travel upward by capillary action. A drug or its metabolite, if there in the oral fluid specimen less than its cut-off concentration, will not saturate the binding spots of its specific antibody. The antibody will next act in response with the drug protein conjugate and a noticeable colored line up will be evidence for the test line region of the explicit drug strip. Presence of drug greater than the cut-off concentration in the oral fluid sample will saturate all the binding spots of the antibody. Therefore, the colored line up will not form in the test line area.

A drug positive oral fluid sample will not produce a colored line in the specific test line area of the strip because of more drug competition, whereas a drug negative oral fluid sample will make a line in the test line area because of the nonexistence of drug competition.

To provide as a technical control, a colored line up will constantly become visible at the control line area, signifying that appropriate volume of sample has been supplied and membrane wicking has taken place.

Tags: Oralert drug test, Oralert fluid drug screen, Oralert oral testing, Oralert testing for drugs, Oratect screening for drugs

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