Barbiturates are the group of drugs that act as central nervous system depressants. These drugs are classified as sedative/hypnotics and produce a wide range of effects from mild sedation to anesthesia. These drugs are medically prescribed to treat insomnia, anxiety, tension, and help in mitigating epileptic seizures. Barbiturate abuse frequently becomes a cause for cases like accidental poisoning and suicide attempts. Certain barbituates are also used to induce surgical anesthesia.

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vulation tests are chromatographic immunoassay used for the detection of a hormone called LH (Luteinizing Hormone). This hormone is present in small quantity and surges in the middle of menstrual cycle. The pituary gland releases the LH in large quantity than any other time in the menstrual cycle. The surge remains for about 3 days and the women are more likely to ovulate at this time. In the initial hours the LH surge is evident in blood, later after 8-12 hours it is detected in urine.

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